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Anti-Fascin Antikörper (A13733)

Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.com Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.com
Knockout-validierter Antikörper
$470
Product Datasheet

Polyklonal Kaninchen Antikörper gegen Fascin für WB, IHC und ICC/IF.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com

Produktname
Anti-Fascin Antibody
Beschreibung
Rabbit polyclonal antibody to Fascin.
Anwendungen
WB, IHC, ICC/IF
Verdünnungen
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reaktivität
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 394-493 of human Fascin/Fascin/FSCN1 (NP_003079.1).
Sequenz
FIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY
Wirt
Rabbit
Klonalität
Polyclonal
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
50 kDa / 55 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
55 kDa actin bundling protein, 55 kDa actin-bundling protein, Actin bundling protein, actin bundling protein, 55-KD, FAN 1, FAN1, Fascin 1, Fascin actin bundling protein 1, Fascin homolog 1, Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus), Fascin, sea urchin, homolog of, 1, Fascin1, FLJ38511, FSCN 1, FSCN1, FSCN1_HUMAN, HSN, p55, Singed (Drosophila) like (sea urchin fascin homolog like), Singed drosophila homolog like, Singed like (fascin homolog sea urchin), Singed like (fascin homolog sea urchin) (Drosophila), Singed like protein, Singed, drosophila, homolog of, Singed-like protein, SNL, Strongylocentrotus purpuratus
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts of various cell lines, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Fascin Antibody (A13733) - Antibodies.comBild Vergrößern
Figure 2: Western Blot - Anti-Fascin Antibody (A13733)
Western blot analysis of extracts from normal (control) and Fascin/Fascin/FSCN1 knockout (KO) HeLa cells, using Anti-Fascin Antibody (A13733) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-Fascin Antibody (A13733) - Antibodies.comBild Vergrößern
Figure 3: Immunohistochemistry - Anti-Fascin Antibody (A13733)
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Fascin Antibody (A13733) - Antibodies.comBild Vergrößern
Figure 4: Immunofluorescence - Anti-Fascin Antibody (A13733)
Immunofluorescence analysis of C6 cells using Anti-Fascin Antibody (A13733) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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