Anti-Gli1 Antikörper [ARC59108] (A309375)

Name: Anti-Gli1 Antibody [ARC59108]
Brand: Antibodies.com
SKU: A309375

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Dieses Produkt wurde eingestellt.

Anti-Gli1 Antikörper [ARC59108] (A309375) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-Gli1 Antikörper anzeigen.

Produktdetails

Produktname

Anti-Gli1 Antibody [ARC59108]

Beschreibung

Rabbit monoclonal [ARC59108] antibody to Gli1.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant protein of human GLI1.

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC59108

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

160 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

GLI, Gli 1, GLI family zinc finger 1, GLI Kruppel family member 1, GLI1_HUMAN, Glioma associated oncogene 1, Glioma associated oncogene homolog, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zfp5, Zinc finger protein GLI 1, Zinc finger protein GLI1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

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Figure 1: Western Blot - Anti-Gli1 Antibody [ARC59108] (A309375)

Western blot analysis of A-549, using Anti-Gli1 Antibody [ARC59108] (A309375) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunohistochemistry analysis of paraffin-embedded mouse colon using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Gli1 Antibody [ARC59108] (A309375)

Immunofluorescence analysis of Neuro-2a cells using Anti-Gli1 Antibody [ARC59108] (A309375) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).