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Anti-His Tag Antikörper [AMC0149] (A17313)

Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.com
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für "His Tag Antikörper"
$230
Product Datasheet

Monoklonal [AMC0149] Maus Antikörper gegen His Tag für WB und IP.

100% Qualitätsgarantie
Preis-Match-Garantie

Versandkosten
$40
Vorlaufzeit
Lieferung in 5-8 Werktagen

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+1 (314) 370-6046
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Produktname
Anti-His Tag Antibody [AMC0149]
Beschreibung
Mouse monoclonal [AMC0149] antibody to His Tag.
Anwendungen
WB, IP
Verdünnungen
WB: 1:2,000-1:20,000, IP: 1:50-1:100
Reaktivität
Species Independent
Immunogen
A synthetic peptide corresponding to His tag.
Sequenz
HHHHHHH
Wirt
Mouse
Klonalität
Monoclonal
Klon
AMC0149
Isotyp
IgG1
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
27 kDa / 35 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
6 His epitope tag, 6X His tag, Hexa His tag, HHHHHH epitope tag, HHHHHH tag, Polyhistidine Tag
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Western blot analysis of various lysates, using Anti-His Tag Antibody [AMC0149] (A17313) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comBild Vergrößern
Figure 2: Immunoprecipitation - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation of over-expressed His-tagged protein in 293T cells incubated using Anti-His Tag Antibody [AMC0149] (A17313). A mock served as negative control and over-expressed 293T cell lysate served as positive control.
Western Blot - Anti-His Tag Antibody [AMC0149] (A17313) - Antibodies.comBild Vergrößern
Figure 3: Western Blot - Anti-His Tag Antibody [AMC0149] (A17313)
Immunoprecipitation analysis of 300µg extract cell lysate from 293T cells transfected with EPCAM expression vector containing a His-tag using 3µg of Anti-His Tag Antibody [AMC0149] (A17313). This Western blot was performed on the immunoprecipitate using Anti-His Tag Antibody [AMC0149] (A17313)at 1:5,000 dilution.

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