Anti-HSP27 Antikörper [6H11] (A85358)

$185

Monoklonal [6H11] Maus Antikörper gegen HSP27 für WB, ICC/IF und IHC.

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Versandkosten

$40

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Lieferung in 4-6 Werktagen

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+1 (314) 370-6046

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Stichworte:

Cytoskeleton Marker

Produktdetails

Produktname

Anti-HSP27 Antibody [6H11]

Beschreibung

Mouse monoclonal (6H11) antibody to HSP27.

Anwendungen

WB, ICC/IF, IHC

Verdünnungen

WB: 1:10,000, ICC/IF: 1,000, IHC: 1:1,000

Reaktivität

Human

Kreuzreaktivität

This antibody does not cross-react with rodent.

Immunogen

Recombinant full-length human HSP27, expressed in and purified from E. coli.

Wirt

Mouse

Klonalität

Monoclonal

Klon

6H11

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Immunogen affinity purification.

Konzentration

1 mg/ml

Molekulargewicht

27 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

28 kDa heat shock protein, CMT2F, DKFZp586P1322, epididymis secretory protein Li 102, Estrogen regulated 24 kDa protein, Estrogen-regulated 24 kDa protein, Heat shock 25kDa protein 1, Heat shock 27 kDa protein, Heat shock 27kD protein 1, Heat shock 27kDa protein, Heat shock 27kDa protein 1, Heat shock 28kDa protein 1, Heat Shock Protein 27, Heat shock protein beta 1, Heat shock protein beta-1, heat shock protein family B (small) member 1, HEL-S-102, HMN2B, HS.76067, Hsp 25, HSP 27, Hsp 28, Hsp B1, Hsp25, Hsp28, HspB1, HSPB1_HUMAN, SRP27, Stress responsive protein 27, Stress-responsive protein 27

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (4)

Figure 1: Immunofluorescence - Anti-HSP27 Antibody [6H11] (A85358)

HeLa cells staining with Anti-Heat Shock Protein 27 Antibody (red), Anti-Vimentin Antibody (A85421 | green) and DNA (blue). The Anti-Heat Shock Protein 27 Antibody reveals strong cytoplasmic staining and penetrates into the actin rich ruffled margins, while the Anti-Vimentin Antibody reveals cytoplasmic intermediate filaments.

Figure 2: Immunofluorescence - Anti-HSP27 Antibody [6H11] (A85358)

Immunofluorescent analysis of HeLa cells stained with Anti-Heat Shock Protein 27 Antibody (1:2,000 | green) and Anti-Heat Shock Protein 60 Antibody (A85438 | 1:2,000 | red). Blue is DAPI staining of nuclear DNA. The Anti-Heat Shock Protein 27 Antibody produces strong cytoplasm staining, while the Anti-Heat Shock Protein 60 Antibody specifically labels mitochondria.

Figure 3: Western Blot - Anti-HSP27 Antibody [6H11] (A85358)

Western blot analysis of tissue or whole cell lysates using Anti-Heat Shock Protein 27 Antibody (1:10,000 | green): [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH/3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. Strong single band at ~27 kDa mark corresponds to HSP27 protein, detected only in human cell lines. The blot was simultaneously probed with Anti-Heat Shock Protein 60 Antibody (A85437 | 1:5,000 | red). A strong 60 kDa band is present in all preparations.

Figure 4: Western Blot - Anti-HSP27 Antibody [6H11] (A85358)

Western blots of HeLa cell crude extracts. Lane 16 was probed with Anti-Heat Shock Protein 27 Antibody, while Lanes 14 and 15 were probed with two other Anti-Heat Shock Protein 27 Antibodies that were generated at the same time (not on the sale). Note the strong clean bands at 27 kDa. Lane 17 was probed with Anti-Lamin A/C Antibody (A85444) which binds two bands running at 74 kDa and 65 kDa. Lane 13 was probed with Anti-Actin Antibody (A85388). Molecular weights of each protein are as indicated, and dots indicate the presence of major HeLa proteins.

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