Anti-HSP90 beta Antikörper [H9010] (A305130)

$520

Monoklonal [H9010] Maus Antikörper gegen HSP90 beta für WB, IHC, ICC/IF, IP, ELISA und Antibody Microarray.

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Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

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+1 (314) 370-6046

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Produktdetails

Produktname

Anti-HSP90 beta Antibody [H9010]

Beschreibung

Mouse monoclonal [H9010] antibody to HSP90 beta.

Spezifität

This antibody detects HSP90 beta specifically in all reactive species - except in Chicken, where it detects both alpha and beta isoforms.

Anwendungen

WB, IHC, ICC/IF, IP, ELISA, Antibody Microarray

Verdünnungen

WB: 1:2500, IHC: 1:100

Reaktivität

Human, Mouse, Rat, Rabbit, Chicken, Canine, Fish, Shark, Hamster

Immunogen

Recombinant human HSP90 beta protein.

Wirt

Mouse

Klonalität

Monoclonal

Klon

H9010

Isotyp

IgG2a

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

90 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

90 kda heat shock protein beta HSP90 beta, D6S182, FLJ26984, Heat shock 84 kDa, Heat shock 90kD protein 1, beta, Heat shock 90kDa protein 1 beta, Heat shock protein 90 alpha family class B member 1, Heat shock protein 90 kDa, Heat shock protein 90kDa alpha (cytosolic) class B member 1, Heat shock protein 90kDa alpha family class B member 1, Heat shock protein beta, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta, HS90B_HUMAN, HSP 84, HSP 90, HSP 90 b, HSP 90b, HSP84, hsp90ab1, HSP90B, HSPC2, HSPCB

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (8)

Figure 1: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Lysates showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2).

Figure 2: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000. The secondary antibody used was HRP Goat Anti-Mouse.

Figure 3: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse backskin, fixed in Bouin's fixative solution and paraffin-embedded. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:100 for 1 hour at room temperature. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at room temperature. Localization: Epidermis.

Figure 4: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 5: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 6: Western Blot - Anti-HSP90 beta Antibody [H9010] (A305130)

Western blot analysis of human cell lysates from various cell lines showing detection of Hsp90 protein using Anti-HSP90 beta Antibody [H9010] (A305130) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

Figure 7: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of human colon carcinoma, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Biotin Goat Anti-Mouse at 1:2000 for 1 hour at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at room temperature. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

Figure 8: Immunohistochemistry - Anti-HSP90 beta Antibody [H9010] (A305130)

Immunohistochemistry analysis of mouse inflamed colon, fixed in formalin. The Primary Antibody used was Anti-HSP90 beta Antibody [H9010] (A305130) at 1:10,000 for 12 hours at 4°C. The secondary antibody used was Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5,000 for 1 hour at room temperature. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications..

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