The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma and other biological fluids.

Sensitivität

4.3 ng/ml

Detektionsbereich

12.5 - 800 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

A2AP, A2AP_HUMAN, AAP, Alpha 2 Antiplasmin, Alpha 2 antiplasmin pigment epithelium derived factor, Alpha 2 AP, ALPHA 2 PI, Alpha 2 plasmin inhibitor deficiency, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-PI, Alpha-2-plasmin inhibitor, Antiplasmin deficiency, API, Plasmin inhibitor deficiency, PLI, Serine (or cysteine) peptidase inhibitor, clade F, member 2, Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_c, Serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2, Serine (Or cysteine) proteinase inhibitor, clade F, member 2, Serine or cysteine peptidase inhibitor clade F member 2, Serpin F2, Serpin peptidase inhibitor clade F, Serpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2, SERPINF2

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.