Assay-Prinzip
This Human ATP13A2 ELISA Kit is an in vitro competitive enzyme-linked immunosorbent assay for the quantitative measurement of ATP13A2 in plasma, tissue homogenates and other biological fluids.
A microplate has been precoated with Anti-ATP13A2 Antibodies. Samples and the ATP13A2-HRP conjugate are added to the wells, where ATP13A2 in the sample competes with the added ATP13A2-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is added. The TMB substrate is catalyzed by HRP to produce blue colouration. The reaction is terminated by addition of Stop Solution which stops the colour development and produces a colour change from blue to yellow. The intensity of the signal is inversely proportional to the amount of ATP13A2 in the sample and the intensity is measured at 45 nm.
