The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Tissue homogenates, cell lysates and other biological fluids.

Sensitivität

0.114 ng/ml

Detektionsbereich

0.312 - 20 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

B2L11_HUMAN, BAM, Bcl 2 interacting protein Bim, Bcl 2 related ovarian death agonist, Bcl-2-like protein 11, BCL2 interacting mediator of cell death, BCL2 like 11, BCL2 like 11 (apoptosis facilitator), Bcl2-interacting mediator of cell death, Bcl2-L-11, Bcl2l11, Bim, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.