The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, tissue homogenates and other biological fluids.

Sensitivität

0.107 ng/ml

Detektionsbereich

0.312 - 20 ng/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Human

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

CEL3A_HUMAN, CELA3A, Chymotrypsin-like elastase family member 3A, ELA1, ELA2A, ELA2B, ELA3, ELA3A, ELA3B, Elastase, Elastase 1, Elastase 2A, Elastase 3A, Elastase 3A pancreatic, Elastase 3B, Elastase 3B pancreatic, Elastase IIIA, Elastase IIIB, Elastase-3A, Fecal pancreatic elastase 1, HLE, Medullasin, Pancreatic elastase IIA, PE 1, PE1, Protease E

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.