Human M-CSF ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of human M-CSF in the samples. A monoclonal antibody specific for human M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and M-CSF present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked detect antibody specific for M-CSF is added to the wells. Following a wash to remove any unbound antibody-biotin reagent, streptavidin-HRP is added. After washing, substrate solution is added to the wells and color develops in proportion to the amount of M-CSF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.19 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Wiederherstellung

102% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant human M-CSF.

Reaktivität

Human

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

Colony stimulating factor 1, Colony stimulating factor 1 (macrophage), Colony stimulating factor macrophage specific, CSF 1, CSF-1, CSF1, CSF1_HUMAN, Csfm, Lanimostim, M CSF, Macrophage colony stimulating factor, Macrophage Colony Stimulating Factor 1, MCSF, MGC31930, Processed macrophage colony-stimulating factor 1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.