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Anti-ICAM1 Antikörper [ARC0261] (A306965)

Western Blot - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.com Immunohistochemistry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.com Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.com Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.com
Dieses Produkt wurde eingestellt.

Anti-ICAM1 Antikörper [ARC0261] (A306965) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-ICAM1 Antikörper anzeigen.

Produktname
Anti-ICAM1 Antibody [ARC0261]
Beschreibung
Rabbit monoclonal [ARC0261] antibody to ICAM1.
Anwendungen
WB, IHC, Flow Cytometry
Verdünnungen
WB: 1:500-1:2,000, IHC: 1:50-1:200, Flow Cytometry: 1:50-1:200
Reaktivität
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human ICAM-1/CD54 (P05362).
Sequenz
MAPSSPRPALPALLVLLGALFPGPGNAQTSVSPSKVILPRGGSVLVTCSTSCDQPKLLGIETPLPKKELLLPGNNRKVYELSNVQEDSQPMCYSNCPDGQ
Wirt
Rabbit
Klonalität
Monoclonal
Klon
ARC0261
Isotyp
IgG
Konjugat

Unconjugated

Reinigung
Affinity purification.
Molekulargewicht
89 kDa / 100 kDa
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyme
Antigen identified by monoclonal, BB 2, BB2, CD 54 , CD54, CD54 antigen , CD_antigen=CD54, Cell surface glycoprotein P3.58 , Human rhinovirus receptor , ICAM 1, ICAM-1, ICAM1_HUMAN, Intercellular adhesion molecule 1 , intercellular adhesion molecule 1 (CD54), human rhinovirus receptor, Major group rhinovirus receptor , MALA 2, MALA2 , MGC6195, MyD 10, MyD10, P3.58 , Surface antigen of activated B cells , Surface antigen of activated B cells, BB2
Isotyp-Kontrollantikörper
Kompatible Sekundärantikörper
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-ICAM1 Antibody [ARC0261] (A306965)
Western blot analysis of extracts of A-549 cells, using Anti-ICAM1 Antibody [ARC0261] (A306965) at 1:1,000 dilution. A-549 cells were treated by TNF-alpha (20 ng/ml) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.comBild Vergrößern
Figure 2: Immunohistochemistry - Anti-ICAM1 Antibody [ARC0261] (A306965)
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-ICAM1 Antibody [ARC0261] (A306965) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.comBild Vergrößern
Figure 3: Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965)
Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-ICAM1 Antibody [ARC0261] (A306965), (10 µg/ml green line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained Raji cells, used as blank control (red line).
Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965) - Antibodies.comBild Vergrößern
Figure 4: Flow Cytometry - Anti-ICAM1 Antibody [ARC0261] (A306965)
Flow cytometry analysis of 293F cells, stained with Rabbit IgG isotype control (10 µg/ml, blue line) or Anti-ICAM1 Antibody [ARC0261] (A306965), (10 µg/ml green line), followed by Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) staining. Non-fluorescently stained 293F cells, used as blank control (red line).

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