Figure 1: Western Blot - Anti-Insulin Receptor (phospho Tyr1150) + IGF1 Receptor (phospho Tyr1135) Antibody (A16411)
Western blot analysis of extracts of 293 and HepG2 cells, using Anti-Insulin Receptor (phospho Tyr1150) + IGF1 Receptor (phospho Tyr1135) Antibody (A16411) at 1:1,000 dilution. 293 cells were treated by EGF (25ug/mL) for 30 minutes after serum-starvation overnight. HepG2 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA.
