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Integrin beta 3 (phospho Tyr785) Cell Based Kit Elisa (A102243)

$645
Versandkosten
Vorlaufzeit
Lieferung in 5-8 Werktagen
Telefon
+1 (314) 370-6046
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Email
orders@antibodies.com
Produktname
Integrin beta 3 (phospho Tyr785) Cell Based ELISA Kit
Assay-Typ
Cell-based (qualitative)
Assay-Prinzip
This Integrin beta 3 (phospho Tyr785) Cell Based ELISA Kit allows for the detection of Integrin beta 3 (phospho Tyr785) and the effects that certain stimulation conditions have on Integrin beta 3 (phospho Tyr785) expression in different cell lines. Qualitative determination of Integrin beta 3 (phospho Tyr785) concentration is achieved by an indirect ELISA format. In essence, the Integrin beta 3 (phospho Tyr785) is captured by Anti-Integrin beta 3 (phospho Tyr785) Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this Integrin beta 3 (phospho Tyr785) Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) Anti-Integrin beta 3 Antibody is provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Adherent cells and suspension cells.
Detektionsbereich
> 5000 Cells
Testdauer
4 hours 30 minutes
Reaktivität
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at + 4°C. Stable for 6 months.
Synonyms
BDPLT16, BDPLT2, CD 61, CD61, CD61 antigen, GP3A, GPIIIa, GT, HPA 1, HPA 4, Integrin beta 3 (platelet glycoprotein IIIa antigen CD61), Integrin beta chain beta 3, Integrin beta-3, ITB3_HUMAN, ITG B3, ITGB 3, ITGB3, NAIT, Platelet fibrinogen receptor beta subunit, Platelet fibrinogen receptor, beta subunit, Platelet glycoprotein IIIa, Platelet glycoprotein IIIa precursor, Platelet membrane glycoprotein IIIa, PTP
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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