Relatlimab Biosimilar - Anti-LAG-3 Antibody - BSA and Azide free
Beschreibung
Recombinant human monoclonal antibody to LAG-3.
Anwendungen
ELISA, Flow Cytometry
Verdünnungen
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reaktivität
Human
Wirt
Human
Klonalität
Monoclonal
Isotyp
IgG4
Konjugat
Unconjugated
Reinigung
Affinity chromatography.
Konzentration
Reconstitution dependent.
Produktform
Lyophilized
Rekonstitution
Reconstitute with distilled sterile water.
Formulierung
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Allgemeine Hinweise
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 1 µg/ml (100 µl/well) Recombinant Human LAG-3 Protein (6xHis Tag) (A318223) which can bind Anti-LAG-3 Antibody [Relatlimab Biosimilar] - Azide free (A318896) in a linear range of 0.6-100 µg/ml.
Flow cytometry analysis with Anti-LAG-3 Antibody [Relatlimab Biosimilar] - Azide free (A318896) at 1 µg/ml on Expi293 cells transfected with human LAG3 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram).
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