This antibody was raised against an intracellular fragment corresponding to amino acids 141-295 of human LIME; a 30 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Anwendungen

IP, Flow Cytometry (Intracellular)

Verdünnungen

Flow Cytometry: 8-12 µg/ml; Intracellular staining.

Reaktivität

Human

Immunogen

Bacterially expressed intracellular fragment corresponding to amino acids 141-295 of human LIME protein.

Wirt

Mouse

Klonalität

Monoclonal

Klon

LIME-06

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein A chromatography.

Konzentration

1 mg/ml

Geschätztes MW

30 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

dJ583P15.4, FLJ20406, Lck interacting membrane protein, Lck interacting molecule, Lck interacting transmembrane adaptor 1, LIME 1, LIME1, LP8067, RP4 583P15.5

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (1)

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Figure 1: Immunoprecipitation - Anti-LIME Antibody [LIME-06] (A86419)

Induction of LIME tyrosine phosphorylation. Peripheral blood T cells were left unstimulated (-) or stimulated with Anti-CD3 Antibody [MEM-92] or Anti-CD4 Antibody [MEM-16], and LIME was immunoprecipitated from laurylmaltoside lysates with Anti-LIME Antibody (A86419) (immunoaffinity sorbent) and analyzed by Western blotting to visualize tyrosine-phosphorylated LIME (top) and total LIME (bottom).