Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Allgemeine Hinweise
This antibody can be used to verify the size of fusion constructs by western blotting, and to amplify the endogenous fluorescence of mCherry in transfected cells.
Immunofluorescent analysis of HEK293 cells transfected with mCherry-HA construct (red) and stained using Anti-mCherry Antibody (A270547), at a dilution of 1:2,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. The Anti-mCherry Antibody reveals mCherry protein is expressed only in transfected cells which appear golden in color. Untransfected cells expressing no mCherry are not recognized by the antibody, so as a result only their nuclei are visible.
Figure 2: Western Blot - Anti-mCherry Antibody (A270547)
Western blot analysis of HEK293 cell lysates using Anti-mCherry Antibody (A270547), at a dilution of 1:2,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] HEK293 control cells, [3] HEK293 cells stably transduced with a lentiviral vector expressing mCherry-HA construct, and [4] HEK293 cells transfected with mCherry-HA construct. The major band at about 30 kDa corresponds to mCherry protein.
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