Anti-MDC1 Antikörper [P2B11] (A304945)

Name: Anti-MDC1 Antibody [P2B11]
Brand: Antibodies.com
SKU: A304945
Price: 465.0 USD
Availability: InStock

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$465

Monoklonal [P2B11] Maus Antikörper gegen MDC1 für WB und ICC/IF.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

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Produktdetails

Produktname

Anti-MDC1 Antibody [P2B11]

Beschreibung

Mouse monoclonal [P2B11] antibody to MDC1.

Spezifität

This antibody recognizes MDC1 at and around the N-terminus.

Anwendungen

WB, ICC/IF

Verdünnungen

WB: 1:2,000, ICC/IF: 1:100

Reaktivität

Human, Mouse, Bovine, Chimpanzee

Immunogen

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Wirt

Mouse

Klonalität

Monoclonal

Klon

P2B11

Isotyp

IgG1

Konjugat

Unconjugated

Reinigung

Protein G purification.

Konzentration

1 mg/ml

Molekulargewicht

~184 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Homologue to Drosophila photoreceptor protein calphotin, MDC 1, MDC1_HUMAN, Mediation of DNA damage checkpoint 1, Mediator of DNA damage checkpoint 1, Mediator of DNA damage checkpoint protein 1, NFBD 1, NFBD1, Nuclear factor with BRCT domains 1, Nuclear Factor with BRCT Domains Protein 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (3)

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Figure 1: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: 293Trap cell lysates. Load: 30 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse HRP: IgG at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 2: Western Blot - Anti-MDC1 Antibody [P2B11] (A304945)

Western blot analysis of mouse Cortex and Cerebellum showing detection of 184 kDa MDC1 protein using Anti-MDC1 Antibody [P2B11] (A304945) at 1:1,000 for 2 hours room temperature. Lane 1: MW ladder. Lane 2: mouse Cortex. Lane 3: mouse Cerebellum. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Mouse at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: 184 kDa.

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Figure 3: Immunofluorescence - Anti-MDC1 Antibody [P2B11] (A304945)

Immunocytochemistry/Immunofluorescence analysis of mouse fibroblast cell line (NIH 3T3), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-MDC1 Antibody [P2B11] (A304945), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:5,000 for 5 minutes room temperature. Localization: Nucleus. Magnification: 60X.