Anti-MLH1 Antikörper [ARC53543] (A308155)

Name: Anti-MLH1 Antibody [ARC53543]
Brand: Antibodies.com
SKU: A308155
Price: 470.0 USD
Availability: InStock

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$470

Monoklonal [ARC53543] Kaninchen Antikörper gegen MLH1 für WB und IHC.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

Kontakt

Telefon

+1 (314) 370-6046

orders@antibodies.com

Garantien

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Stichworte:

Knockout-validierter Antikörper

Produktdetails

Produktname

Anti-MLH1 Antibody [ARC53543]

Beschreibung

Rabbit monoclonal [ARC53543] antibody to MLH1.

Anwendungen

WB, IHC

Verdünnungen

WB: 1:10,000-1:70,000, IHC: 1:50-1:200

Reaktivität

Human, Mouse

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1 (NP_000240.1).

Sequenz

PGLAGPSGEMVKSTTSLTSSSTSGSSDKVYAHQMVRTDSREQKLDAFLQPLSKPLSSQPQAIVTEDKTDISSGRARQQDEEMLELPAPAEVAAKNQSLEGD

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC53543

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

85 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

COCA 2, COCA2, DNA mismatch repair protein Mlh1, FCC 2, FCC2, hMLH 1, hMLH1, HNPCC, HNPCC 2, HNPCC2, MGC5172, MLH 1, MLH1_HUMAN, MutL homolog 1, MutL homolog 1 (E. coli), MutL homolog 1 colon cancer nonpolyposis type 2, MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli), MutL protein homolog 1, MutL, E. coli, homolog of, 1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of various cell lines, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts of mouse brain, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Western Blot - Anti-MLH1 Antibody [ARC53543] (A308155)

Western blot analysis of extracts from normal (control) and MLH1 knockout (KO) HeLa cells, using Anti-MLH1 Antibody [ARC53543] (A308155) at 1:60,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 4: Immunohistochemistry - Anti-MLH1 Antibody [ARC53543] (A308155)

Immunohistochemistry analysis of paraffin-embedded human colon cancer loss of MLH1 expression (negative control sample) using Anti-MLH1 Antibody [ARC53543] (A308155) at a dilution of 1:200(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.