This Fractalkine enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-Fractalkine Antibody. Standards or samples are then added to the microtire plate wells and Fractalkine, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of Fractalkine present in the sample, Anti-Fractalkine Antibody (Biotin) is added to each well to "sandwich" the Fractalkine immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain Fractalkine will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, body fluids, tissue lysates, and cell culture supernatants.

Sensitivität

< 10 pg/ml

Detektionsbereich

156-10,000 pg/ml

Reaktivität

Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2-8°C for 4 months or at -20°C for 8 months.

Synonyms

A 152E5.2, AB030188, ABCD 3, ABCD3, AI848747, C X3 C motif chemokine 1, C-X3-C motif chemokine 1, C3Xkine, Chemokine (C X3 C motif) ligand 1, Chemokine (C-X3-C motif) ligand 1, Chemokine C X3 C motif ligand 1, Chemokine CX3C Motif Ligand 1, CX3C membrane anchored chemokine, CX3C membrane-anchored chemokine, CX3CL1, CXC 3, CXC3, CXC3C, D8Bwg0439e, FKN, Neurotactin, NTN, NTT, Processed fractalkine, SCYD 1 , SCYD1, Small inducible cytokine D1, Small inducible cytokine subfamily D (Cys X3 Cys) member 1, Small inducible cytokine subfamily D (Cys X3 Cys), member 1, Small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin) , Small inducible cytokine subfamily D member 1, Small-inducible cytokine D1, X3CL1_HUMAN

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.