This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to the index has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled the index and unlabeled the index (Standards or samples) with the pre-coated antibody specific to the index. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of the index in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of the index in the sample.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivität
17.34 pg/ml
Detektionsbereich
23.437 - 1500 pg/ml
Testdauer
1 - 4.5 Hours
Reaktivität
Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Synonyms
GCG
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
