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Rekombinant Anti-Maus IgG2b Antikörper [RM108] (Biotin) (A121264)

Western Blot - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.com ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.com ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121332) - Antibodies.com
$480

Anti-Maus IgG2b Antikörper [RM108] (Biotin) für IP, ICC, IHC, Flow Cytometry, ELISA und WB.

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Versandkosten
$40
Vorlaufzeit
Lieferung in 4-6 Werktagen

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+1 (314) 370-6046
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Produktname
Anti-Mouse IgG2b Antibody [RM108] (Biotin)
Beschreibung
Recombinant rabbit monoclonal [RM108] antibody to Mouse IgG2b (Biotin).
Wirt
Rabbit
Zielarten
Mouse
Spezifität
This antibody recognizes the Fc region of mouse IgG2b.
Anwendungen
IP, ICC, IHC, Flow Cytometry, ELISA, WB
Verdünnungen
ELISA: 0.005-0.2 µg/ml, ICC: 0.5-2 µg/ml, IHC: 0.5-2 µg/ml, WB: 0.10-5 µg/ml
Immunogen
Mouse IgG2b.
Antigen-Spezies
Mouse
Klonalität
Monoclonal
Isotyp
IgG
Konjugat

Biotin

Produktform
Liquid
Konzentration
1 mg/ml
Reinigung
Protein A chromatography.
Formulierung
Supplied in Phosphate Buffered Saline with 50% Glycerol, 1% BSA, and 0.09% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Western Blot - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.comBild Vergrößern
Figure 1: Western Blot - Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
Western blot of non-reduced (-) and reduced (+) mouse IgG2b (20ng/lane), using 0.2µg/ml of Anti-Mouse IgG2b Antibody [RM108] (Biotin). This antibody reacts to non-reduced IgG2b (~150 kDa) much stronger than the reduced ?2b form (~50 kDa).
ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121264) - Antibodies.comBild Vergrößern
Figure 2: Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
ELISA of mouse immunoglobulins shows Anti-Mouse IgG2b Antibody [RM108] reacts to the Fc region of mouse IgG2b. There is no cross reactivity with IgG1, IgG2a, IgG3, IgM, IgA, IgE, human IgG, rat IgG, or goat IgG. The plate was coated with 50ng/well of different immunoglobulins. 200ng/ml, 50ng/ml, or 10ng/ml of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
ELISA - Anti-Mouse IgG2b Antibody (Biotin) [RM108] (A121332) - Antibodies.comBild Vergrößern
Figure 3: Anti-Mouse IgG2b Antibody [RM108] (Biotin) (A121264)
A titer ELISA of mouse IgG2b. The plate was coated with different amounts of mouse IgG2b. A serial dilution of Anti-Mouse IgG2b Antibody [RM108] was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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