Mouse IL-23 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse IL-23 in the samples. A monoclonal antibody specific for mouse IL-23 has been immobilized onto microwells. Standard or samples are pipetted into the wells, and IL-23 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-linked monoclonal antibody specific for IL-23 is added to the wells. Following a wash to remove any unbound detect antibody, streptavidin-HRP is added. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of IL-23 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

3.10 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

91% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant mouse IL-23.

Reaktivität

Mouse

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

IL 23 A, IL 23 subunit alpha, IL 23A, IL 23p19, IL-23 subunit alpha, IL-23-A, IL-23p19, IL12B, IL23, Il23a, IL23A_HUMAN, IL23P19, interleukin 12B, Interleukin 23 alpha subunit p19, Interleukin 23 p19 subunit, interleukin 23 subunit alpha, interleukin 23 subunit p19, interleukin six, G CSF related factor, Interleukin-23 subunit alpha, Interleukin-23 subunit p19, JKA3 induced upon T cell activation, MGC79388, P19, SGRF

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.