The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, urine and other biological fluids.

Sensitivität

25.5 pg/ml

Detektionsbereich

62.5 - 4000 pg/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Mouse

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

16.3A5, 1F5, 1F5 antigen, 20 kDa homologous restriction factor, CD 59, CD59, CD59 antigen, CD59 antigen complement regulatory protein , CD59 antigen p18 20, CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344), CD59 glycoprotein, CD59 molecule, CD59 molecule complement regulatory protein, Cd59a, CD59_HUMAN, CD_antigen=CD59, Complement regulatory protein, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, HRF 20, HRF-20, HRF20, Human leukocyte antigen MIC11, Ly 6 like protein, Lymphocytic antigen CD59/MEM43, MAC inhibitory protein, MAC IP, MAC-inhibitory protein, MAC-IP, MACIF, MACIP, MEM43, MEM43 antigen, Membrane attack complex (MAC) inhibition factor, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MGC2354, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18 20, Surface antigen recognized by monoclonal, T cell activating protein

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.