Figure 1: Western Blot - Anti-MSH2 Antibody (A13256)
Western blot analysis of extracts of various cell lines, using Anti-MSH2 Antibody (A1121) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Immunoprecipitation analysis of 150µg extracts of A549 cells using 3µg Anti-MSH2 Antibody (A1121). Western blot was performed from the immunoprecipitate using Anti-MSH2 Antibody (A1121) at a dilition of 1:1000.
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