This antibody recognises the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork through its interaction with FEN1. PCNA is the auxilliary protein for DNA polymerase d. PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse , chicken (Franz-Odendaal 2008) and abalone.
Anwendungen
Flow Cytometry
Verdünnungen
FC 1: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Rat PCNA made in the protein A expression vector pR1T2T.
Wirt
Mouse
Klonalität
Monoclonal
Klon
PC10
Isotyp
IgG2a
Konjugat
FITC
Excitation: 490nm, Emission: 525nm
Reinigung
Protein G affinity chromatography of tissue culture supernatant.
Konzentration
100 µg/ml
Produktform
Liquid
Formulierung
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Lagerung
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Allgemeine Hinweise
Mouse anti PCNA antibody, clone PC10 recognizes the proliferating cell nuclear antigen, also known as PCNA or cyclin. PCNA is a 261 amino acid ~28 kDa nuclear protein vital for cellular DNA synthesis at the replication fork (Li et al. 1995) through its interaction with FEN1 (Wu et al. 1996). PCNA is the auxilliary protein for DNA polymerase d (Bravo et al. 1987).PCNA is highly conserved between mammalian species and other vertebrates. Mouse anti PCNA antibody, clone PC10 has been used for the detection of PCNA in a number of species including human, rat, mouse (Park et al. 2008), chicken (Franz-Odendaal 2008) and abalone (Harris et al. 2006).