Anti-PHD3 Antikörper [ARC52452] (A307618)

Name: Anti-PHD3 Antibody [ARC52452]
Brand: Antibodies.com
SKU: A307618

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Dieses Produkt wurde eingestellt.

Anti-PHD3 Antikörper [ARC52452] (A307618) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-PHD3 Antikörper anzeigen.

Produktdetails

Produktname

Anti-PHD3 Antibody [ARC52452]

Beschreibung

Rabbit monoclonal [ARC52452] antibody to PHD3.

Anwendungen

WB, IHC, ICC/IF

Verdünnungen

WB: 1:2,000-1:20,000, IHC: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PHD3 (NP_071356.1).

Sequenz

MPLGHIMRLDLEKIALEYIVPCLHEVGFCYLDNFLGEVVGDCVLERVKQLHCTGALRDGQLAGPRAGVSKRHLRGDQITWIGGNEEGCEAISFLLSLIDR

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC52452

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

32 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

Egl 9 family hypoxia inducible factor 3, Egl nine homolog 3, Egl nine homolog 3 (C. elegans), Egl nine like protein 3 isoform, EGL9 homolog of C. elegans 3, EGLN3, EGLN3_HUMAN, Factor responsive smooth muscle protein, HIF Prolyl Hydroxylase 3, HIF-PH3, HIF-prolyl hydroxylase 3, HIFP4H3, HIFPH3, HPH-1, HPH-3, Hypoxia-inducible factor prolyl hydroxylase 3, P4H3, Prolyl Hydroxylase Domain Containing Protein 3, Prolyl hydroxylase domain-containing protein 3, SM20

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (7)

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Figure 1: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of various cell lines, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Figure 2: Western Blot - Anti-PHD3 Antibody [ARC52452] (A307618)

Western blot analysis of extracts of BxPC-3 cells, using Anti-PHD3 Antibody [ARC52452] (A307618) at 1:20,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-PHD3 Antibody [ARC52452] (A307618)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-PHD3 Antibody [ARC52452] (A307618) at a dilution of 1:1,000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.