TopBP1 ist ein Gen, das durch das Symbol TOPBP1 kodiert wird. Es ist auch bekannt als: DNA topoisomerase 2-binding protein 1; DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; KIAA0259. TopBP1 hat eine Masse von 170.68kDa und eine Aminosäurelänge von 1522.
Wir bieten 8 antikörper gegen TopBP1, aufgewachsen in Kaninchen und Maus, welche geeignet sind für WB, IHC, ICC/IF and IP mit Proben abgeleitet von Human, Maus und Ratte.
Gen- und Proteininformationen
UniProt Zusammenfassung
Required for DNA replication. Plays a role in the rescue of stalled replication forks and checkpoint control. Binds double-stranded DNA breaks and nicks as well as single-stranded DNA. Recruits the SWI/SNF chromatin remodeling complex to E2F1-responsive promoters. Down-regulates E2F1 activity and inhibits E2F1-dependent apoptosis during G1/S transition and after DNA damage. Induces a large increase in the kinase activity of ATR (PubMed:16530042).
Entrez Zusammenfassung
This gene encodes a binding protein which interacts with the C-terminal region of topoisomerase II beta. This interaction suggests a supportive role for this protein in the catalytic reactions of topoisomerase II beta through transient breakages of DNA strands.
Gewebespezifität
Highly expressed in heart, brain, placenta, lung and kidney.
Posttranslationale Modifikation
Phosphorylated on serine and threonine residues in response to X-ray irradiation.
Zellort
Nucleus. Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome. Cytoplasm > Cytoskeleton > Spindle pole. Chromosome.
Detected on unpaired autosomes in meiotic prophase cells. Detected on X and Y chromosomes during later stages of prophase. Colocalizes with ATR and H2AX at unsynapsed chromosome cores during prophase (By similarity). Has a uniform nuclear distribution during G phase. Colocalizes with BRCA1 at stalled replication forks during S phase. In mitotic cells it colocalizes with BRCA1 at spindle poles and centrosomes during metaphase and anaphase. Detected in discrete foci together with PML and numerous DNA repair enzymes after DNA damage by alkylating agents, UV or gamma irradiation. Localizes to sites of DNA damage in a H2AX- independent manner.
