Anti-PTEN Antikörper [ARC2643] (A307897)

Name: Anti-PTEN Antibody [ARC2643]
Brand: Antibodies.com
SKU: A307897

Bild Vergrößern

Dieses Produkt wurde eingestellt.

Anti-PTEN Antikörper [ARC2643] (A307897) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-PTEN Antikörper anzeigen.

Produktdetails

Produktname

Anti-PTEN Antibody [ARC2643]

Beschreibung

Rabbit monoclonal [ARC2643] antibody to PTEN.

Anwendungen

WB, IHC, ICC/IF, IP

Verdünnungen

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human PTEN (P60484).

Sequenz

MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC2643

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

54 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

10q23del, BZS, DEC, GLM2, MGC11227, MHAM, MMAC1, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, Phosphatase and tensin like protein, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN1, PTEN_HUMAN, TEP1

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (6)

Bild Vergrößern

Figure 1: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of various cell lines, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Bild Vergrößern

Figure 2: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts of C6 cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Bild Vergrößern

Figure 3: Western Blot - Anti-PTEN Antibody [ARC2643] (A307897)

Western blot analysis of extracts from wild type (WT) and PTEN knockout (KO) HeLa cells, using Anti-PTEN Antibody [ARC2643] (A307897) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

Bild Vergrößern

Figure 4: Immunohistochemistry - Anti-PTEN Antibody [ARC2643] (A307897)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-PTEN Antibody [ARC2643] (A307897) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.