Assay-Prinzip
This Rabbit MUC5AC ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rabbit MUC5AC in plasma, tissue homogenates and other biological fluids.
An antibody specific for MUC5AC has been coated on a microplate. Standards and samples are pipetted into the wells and the MUC5AC present in each sample is bound to the wells by the immobilised antibody. The wells are washed and Anti-MUC5AC Antibody (Biotin) is added. After washing away the unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are washed again, before a TMB substrate solution is added to the wells and colour develops in proportion to the amount of MUC5AC bound. The Stop Solution changes the colour from blue to yellow, and the intensity of the colour is measured at 450 nm.
