Rat CXCL1 ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of rat CXCL1 in the samples. A monoclonal antibody specific for rat CXCL1 has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for CXCL1, and CXCL1 present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of CXCL1 bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Cell culture supernatant, serum and plasma.

Sensitivität

0.26 pg/ml

Detektionsbereich

15.63 - 1000 pg/ml

Wiederherstellung

100% (Average Recovery from Serum Samples).

Spezifität

This kit can assay both natural and recombinant rat CXCL1.

Reaktivität

Rat

Kreuzreaktivität

This assay has shown no significant cross-reactivity.

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Lagerung

Store at 2 - 8°C.

Synonyms

C-X-C motif chemokine 1, chemokine (C-X-C motif) ligand 1, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha), CINC-1, Cytokine-induced neutrophil chemoattractant 1, fibroblast secretory protein, FSP, GRO, Gro 1, Gro A, GRO alpha, GRO protein, alpha, GRO-alpha(1-73), GRO-alpha(6-73), GRO1, GRO1 oncogene, GRO1 oncogene (melanoma growth stimulating activity, alpha), GRO1 oncogene (melanoma growth-stimulating activity), GROa, GROA_HUMAN, growth-regulated alpha protein, KC, KC chemokine, mouse, homolog of, Keratinocyte-derived chemokine, mouse, homolog of, Melanoma growth stimulatory activity, melanoma growth stimulatory activity alpha, Melanoma growth stimulatory activity, alpha, MGSA, MGSA a, MGSA alpha, MGSA-a, N51, NAP 3, NAP-3, NAP3, Neutrophil-activating protein 3, Platelet-derived growth factor-inducible protein KC, Scyb 1, SCYB1, Secretory protein N51, Small inducible cytokine subfamily B, member 1

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.