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Ratte GDNF Kit Elisa (A577)

$635
Versandkosten
Vorlaufzeit
Lieferung in 7-10 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
Rat GDNF ELISA Kit
Assay-Typ
Sandwich (quantitative)
Assay-Prinzip
This GDNF enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-GDNF Antibody. Standards or samples are then added to the microtire plate wells and GDNF, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of GDNF present in the sample, Anti-GDNF Antibody (Biotin) is added to each well to "sandwich" the GDNF immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GDNF will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, body fluids, tissue lysates, and cell culture supernatants.
Sensitivität
< 4 pg/ml
Detektionsbereich
31.2-2000 pg/ml
Reaktivität
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
Astrocyte derived trophic factor, Astrocyte derived trophic factor 1, Astrocyte-derived trophic factor, ATF, ATF 1, ATF 2, ATF1, ATF2, GDNF_HUMAN, Glial cell derived neurotrophic factor, Glial cell line derived neurotrophic factor , Glial cell line-derived neurotrophic factor, Glial derived neurotrophic factor, HFB1 GDNF, hGDNF, HSCR3
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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