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Ratte IL4 Kit Elisa (A612)

$615
Versandkosten
Vorlaufzeit
Lieferung in 7-10 Werktagen
Telefon
+1 (314) 370-6046
Mo bis Frei, 8am - 4pm AST
Email
orders@antibodies.com
Produktname
Rat IL4 ELISA Kit
Assay-Typ
Sandwich (quantitative)
Assay-Prinzip
This IL4 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate has been pre-coated with an Anti-IL4 Antibody. Standards or samples are then added to the microtire plate wells and IL4, if present, will bind to the antibody pre-coated on the wells. In order to quantify the amount of IL4 present in the sample, Anti-IL4 Antibody (Biotin) is added to each well to "sandwich" the IL4 immobilised on the plate. The microtiter plate then undergoes incubation, followed by thorough washing of the wells to remove all unbound components. Next, Avidin-Biotin-Peroxidase Complex is added to each well for a short incubation period, followed by thorough washing of the wells to remove all unbound conjugates. Next, a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL4 will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a stop solution and the colour change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
Plattform
Microplate
Nachweismethode
Colorimetric
Probentyp
Serum, body fluids, tissue lysates, and cell culture supernatants.
Sensitivität
< 1 pg/ml
Detektionsbereich
15.6-1000 pg/ml
Reaktivität
Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Lagerung
Store at 2-8°C for 4 months or at -20°C for 8 months.
Synonyms
B cell growth factor 1, B cell IgG differentiation factor, B cell stimulatory factor 1 , B-cell stimulatory factor 1, BCGF 1, BCGF1, Binetrakin , BSF 1, BSF-1, BSF1 , IGG1 induction factor, IL 4, IL-4, Il4e12, IL4_HUMAN, Interleukin 4, Interleukin 4 variant 2, Interleukin 4, isoform 1, Interleukin-4, Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

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