The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Plattform

Microplate

Nachweismethode

Colorimetric

Probentyp

Serum, plasma, tissue homogenates and other biological fluids.

Sensitivität

13.3 pg/ml

Detektionsbereich

31.25 - 2000 pg/ml

Testdauer

1 - 4.5 Hours

Reaktivität

Rat

Precision

Intra-assay CV: < 8%, Inter-assay CV: < 10%

Synonyms

Atriopeptidase, CALLA, CD10, CD10 antigen, Common acute lymphocytic leukemia antigen, DKFZp686O16152, EC 3.4.24.11, Enkephalinase, EPN, Membrane metallo endopeptidase, Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase), Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10), Membrane metallo endopeptidase variant 1, Membrane metallo endopeptidase variant 2, Membrane metalloendopeptidase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase, Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10, Membrane metalloendopeptidase variant 1, Membrane metalloendopeptidase variant 2, MGC126681, MGC126707, MME, NEP, neprilysin-390, neprilysin-411, NEP_HUMAN, Neutral endopeptidase, Neutral endopeptidase 24.11, Neutral endopeptidase, membrane-associated, SFE, Skin fibroblast elastase

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.