Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human OR52D1 protein to be purified and analysed in vitro.
Ausdruckssystem
HEK293 cells
Natur
Synthetic
Spezies
Human
Proteinlänge
Full length protein.
Molekulargewicht
Full length human OR52D1 protein has a MW of 34.9 kDa.
Konjugat
Unconjugated
Produktform
Lyophilized
Konzentration
Reconstitution dependent.
Formulierung
Lyophilized from nanodisc solubilization buffer (20mM Tris-HCl, 150mM NaCl, pH 8.0). Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Lagerung
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
Synonyme
HOR 5'Beta14, O52D1_HUMAN, Odorant receptor HOR5''beta14, Olfactory receptor 52D1, Olfactory receptor family 52 subfamily D member 1, Olfactory receptor OR11-43, OR11-43
Haftungsausschluss
Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.
Figure 1: Synthetic Nanodisc Human OR52D1 Protein (A317294)
ELISA plates were pre-coated with Synthetic Nanodisc Human OR52D1 Protein (A317294) at 0.2µg/per well. Serial diluted Anti-Flag Monoclonal Antibody solutions were added, washed, and incubated with a secondary antibody before reading the ELISA. From the above data, the EC50 for Anti-Flag Monoclonal Antibody binding with Synthetic Nanodisc Human OR52D1 Protein (A317294) is 3.737ng/ml.
Figure 2: Western Blot - Synthetic Nanodisc Human OR52D1 Protein (A317294)
Western Blot analysis of Synthetic Nanodisc Human OR52D1 Protein (A317294) with Anti-Flag Monoclonal Antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution.
