The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Produktform

1mg/ml in PBSwith0.1%SodiumAzide,50%Glycerol.

Synonyme

CSC 21K , CSC-21K, CSC21K , D11Bwg1104e, DDC8, Metalloproteinase inhibitor 2 , Metalloproteinase inhibitor 2 precursor , MGC105282, OTTMUSP00000004170, OTTMUSP00000004171, RP23-394O9.2, TIMP 2 , TIMP metallopeptidase inhibitor 2 , TIMP-2, TIMP2_HUMAN, Tissue inhibitor of metalloproteinase 2, Tissue inhibitor of metalloproteinases 2

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

Bild Vergrößern

Figure 1: Anti-TIMP2 Antibody (BS6747)

For details please refer to datasheet.

Bild Vergrößern

Figure 2: Anti-TIMP2 Antibody (BS6747)

For details please refer to datasheet.

Produktreferenzen (2)

Publikation anzeigen

Gleditsioside B, a triterpene saponin isolated from the anomalous fruits of Gleditsia sinensis Lam., abrogates bFGF-induced endothelial cell migration through preventing the activation of MMP-2 and FAK via inhibiting ERK and PI3K/AKT signaling pathways.

Verweise: Anti-TIMP2 Antibody (A30000)

Abstrakt:

Angiogenesis has become an attractive target for the treatment of certain diseases such as cancer and rheumatoid arthritis. Our previous studies demonstrated that the saponin fraction from Gleditsia sinensis fruits had anti-angiogenic potential, and Gleditsiosides B (GB) was probably the main active constituent. In the present study, we assessed the effect of GB on endothelial cell migration, a crucial event in angiogenesis, and explored the underlying mechanisms. The migration of endothelial cells was assessed by transwell. The expressions of MMP-2/-9 and TIMP-1/-2 were analyzed by Western blotting, and the activities of MMP-2/-9 were detected by gelatin zymography assay. Moreover, migration-related proteins and signaling pathways, including FAK, MAPKs and PI3K/AKT, were analyzed by Western blotting. It was shown that GB, at a concentration of 10 μM without significant cytotoxicity, could effectively abrogate the migration of human umbilical vein endothelial cells (HUVECs) induced by bFGF. GB also inhibited the expression and activity of MMP-2, elevated the expression of TIMP-1, and restrained the phosphorylations of FAK, ERK, PI3K and AKT in a concentration-dependent manner. The findings suggest that GB was able to abrogate the migration of endothelial cells through down-regulating the activation of MMP-2 and FAK via preventing ERK and PI3K/AKT signaling pathways.

Publikation anzeigen

SiRNA directed against annexin II receptor inhibits angiogenesis via suppressing MMP2 and MMP9 expression.

Verweise: Anti-TIMP2 Antibody (A30000)

Abstrakt:

BACKGROUND/AIMS:

Annexin II receptor (AXIIR) is able to mediate Annexin II signal and induce apoptosis, but its role in angiogenesis remains unclear. This study tries to investigate the role of AXIIR in angiogenesis and the plausible molecular mechanism.

METHODS/RESULTS:

RNA interference technology was used to silence AXIIR, and the subsequent effects in vitro and in vivo were evaluated thereafter. Our data indicated that human umbilical vein endothelial cells (HUVECs) expressed AXIIR and knockdown of AXIIR significantly inhibited HUVECs proliferation, adhesion, migration, and tube formation in vitro and suppressed angiogenesis in vivo. Furthermore, AXIIR siRNA induced cell arrest in the S/G2 phase while had no effect on cell apoptosis. We found that these subsequent effects might be via suppressing the expression of matrix metalloproteinase 2and matrix metalloproteinase 9.

CONCLUSION:

AXIIR participates in angiogenesis, and may be a potential therapeutic target for angiogenesis related diseases.

Publikation anzeigen