Anti-V5 Tag Antikörper (A121614)

Name: Anti-V5 Tag Antibody
Brand: Antibodies.com
SKU: A121614
Price: 535.0 USD
Availability: InStock

1 Produktreferenzen

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$535

Polyklonal Ziege Antikörper gegen V5 Tag für WB.

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Versand

Versandkosten

$40

Vorlaufzeit

Lieferung in 5-8 Werktagen

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Produktdetails

Produktname

Anti-V5 Tag Antibody

Beschreibung

Goat polyclonal antibody to V5 Tag.

Spezifität

The antibody recognizes the V5 tag fused to either the amino- or carboxy-terminal of recombinant proteins.

Anwendungen

Verdünnungen

WB: 1:500-1:5,000, IF: 1:500-1:2,000

Immunogen

Recombinant peptide corresponding to amino acids 95-108 of the P and V proteins of the Paramyxovirus of simian virus SV5, expressed in and purified from E. coli.

Sequenz

GKPIPNPLLGLDST

Wirt

Goat

Klonalität

Polyclonal

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Konzentration

3 mg/ml

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline with 20% Glycerol and 0.05% Sodium Azide.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyme

GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, rev, V5 epitope tag

Isotyp-Kontrollantikörper

Goat IgG (A121671)

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (2)

Bild Vergrößern

Figure 1: Western Blot - Anti-V5 Tag Antibody (A121614)

MBP-V5 recombinant protein and 293 cells, transfected with V5-mCherry, detected with Anti-V5 Tag Antibody at a 1:1,000 dilution. Lysates at 100µg per lane and rabbit anti-goat IgG antibody (HRP) at a 1:10,000 dilution.

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Figure 2: Anti-V5 Tag Antibody (A121614)

Myo5a deficient melanocytes transiently transduced with adenovirus expressing V5-tagged Myo5a, fixed, and stained with Anti-V5 Tag Antibody at a 1:1,000 dilution. Donkey anti-goat IgG antibody (Alexa Fluor 568) was used as the secondary antibody.

Produktreferenzen (1)

Publikation anzeigen

The Na⁺ -coupled glucose transporter SGLT2 interacts with its accessory unit MAP17 in vitro and their expressions overlap in the renal proximal tubule.

Verweise: Anti-V5 Tag Antibody (A121614)

Abstrakt:

Na⁺ -glucose cotransporter 2 is the renal Na⁺ -coupled glucose transporter responsible for the tubular glucose reabsorption, while MAP17 was recently identified as its accessory unit. Mutations in either of the proteins' coding genes, SLC5A2 and PDZK1IP1, lead to urinary glucose excretion. To investigate whether MAP17 interacts with SGLT2 in vitro, we engineered a V5-tagged SGLT2 construct and evaluated HEK293T cells coexpressing it together with a HA tagged MAP17 construct. MAP17 is shown to colocalize and coimmunoprecipitate with SGLT2. Also, in human kidney sections, the expression of both proteins overlaps at the apical surface of tubular epithelia. This interaction provides the rationale behind SGLT2 activation by MAP17 as well the similarity of the SLC5A2 and PDZK1IP1 glucosuric phenotypes.

Publikation anzeigen