This antibody recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Anwendungen
WB, IHC-P
Verdünnungen
WB: 1-2 µg/ml, IHC-P: 0.25-0.5 µg/ml
Reaktivität
Human
Immunogen
Recombinant fragment, around amino acids 179-311, of human Villin protein. The exact sequence is proprietary.
Wirt
Mouse
Klonalität
Monoclonal
Klon
VIL1/1314
Isotyp
IgG1
Leichte Ketten
kappa
Konjugat
Unconjugated
Reinigung
Protein A/G chromatography.
Konzentration
200 µg/ml
Molekulargewicht
93 kDa
Produktform
Liquid
Formulierung
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Lagerung
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
SDS-PAGE analysis of Anti-Villin Antibody [VIL1/1314] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Alternative Produkte zum Anti-Villin Antikörper [VIL1/1314] (A250296)