Anti-YAP1 Antikörper [ARC53469] (A309959)

Name: Anti-YAP1 Antibody [ARC53469]
Brand: Antibodies.com
SKU: A309959

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Dieses Produkt wurde eingestellt.

Anti-YAP1 Antikörper [ARC53469] (A309959) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-YAP1 Antikörper anzeigen.

Produktdetails

Produktname

Anti-YAP1 Antibody [ARC53469]

Beschreibung

Rabbit monoclonal [ARC53469] antibody to YAP1.

Anwendungen

WB, IP, ChIP

Verdünnungen

WB: 1:2,000-1:4,000, IP: 1:50-1:200, CUT&Tag: 1⁶ cells/1µg, CHIP: 1:50-1:200

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant protein of human YAP1.

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC53469

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

70 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

65 kDa Yes associated protein, 65 kDa Yes-associated protein, COB1, YAP, YAp 1, YAP 65, YAP1_HUMAN, YAP2, YAP65, yes -associated protein delta, Yes associated protein 1, Yes associated protein 1 65kDa, Yes associated protein 2, yes associated protein beta, YKI, Yorkie homolog

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (5)

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Figure 1: Anti-YAP1 Antibody [ARC53469] (A309959)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 1µg of Anti-YAP1 Antibody [ARC53469] (A309959), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of YAP1 in representative gene loci (PTBP1), as shown in figure.

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Figure 2: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts of C6, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts of Mouse heart, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 4: Western Blot - Anti-YAP1 Antibody [ARC53469] (A309959)

Western blot analysis of extracts from wild type(WT) and YAP1 knockout (KO) HeLa cells, using Anti-YAP1 Antibody [ARC53469] (A309959) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.