Anti-YAP1 Antikörper [ARC53479] (A308259)

Name: Anti-YAP1 Antibody [ARC53479]
Brand: Antibodies.com
SKU: A308259

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Dieses Produkt wurde eingestellt.

Anti-YAP1 Antikörper [ARC53479] (A308259) wurde eingestellt und ist nicht mehr verfügbar.

Alle Anti-YAP1 Antikörper anzeigen.

Produktdetails

Produktname

Anti-YAP1 Antibody [ARC53479]

Beschreibung

Rabbit monoclonal [ARC53479] antibody to YAP1.

Anwendungen

WB, IHC, ICC/IF, IP, ChIP

Verdünnungen

WB: 1:2,000-1:4,000, IHC: 1:50-1:200, IP: 1:1,000-1:5,000, ICC/IF: 1:50-1:200, ChIP: 1:20-1:50

Reaktivität

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).

Sequenz

PTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL

Wirt

Rabbit

Klonalität

Monoclonal

Klon

ARC53479

Isotyp

IgG

Konjugat

Unconjugated

Reinigung

Affinity purification.

Molekulargewicht

73 kDa

Produktform

Liquid

Formulierung

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Lagerung

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyme

65 kDa Yes associated protein, 65 kDa Yes-associated protein, COB1, YAP, YAp 1, YAP 65, YAP1_HUMAN, YAP2, YAP65, yes -associated protein delta, Yes associated protein 1, Yes associated protein 1 65kDa, Yes associated protein 2, yes associated protein beta, YKI, Yorkie homolog

Isotyp-Kontrollantikörper

Kompatible Sekundärantikörper

Haftungsausschluss

Dieses Produkt ist nur für Forschungszwecke bestimmt. Es ist nicht für diagnostische oder therapeutische Zwecke bestimmt.

Produktbilder (9)

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Figure 1: Western Blot - Anti-YAP1 Antibody [ARC53479] (A308259)

Western blot analysis of extracts from normal (control) and YAP1 knockout (KO) HeLa(KO) cells, using Anti-YAP1 Antibody [ARC53479] (A308259) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Western Blot - Anti-YAP1 Antibody [ARC53479] (A308259)

Western blot analysis of C6, using Anti-YAP1 Antibody [ARC53479] (A308259) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Western Blot - Anti-YAP1 Antibody [ARC53479] (A308259)

Western blot analysis of Mouse heart, using Anti-YAP1 Antibody [ARC53479] (A308259) at 1:4,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

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Figure 4: Immunohistochemistry - Anti-YAP1 Antibody [ARC53479] (A308259)

Immunohistochemistry analysis of paraffin-embedded human colon tissue (low expression samples) using Anti-YAP1 Antibody [ARC53479] (A308259) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.