Anti-Actin Anticorps [5J11] (A85388)

Name: Anti-Actin Antibody [5J11]
Brand: Antibodies.com
SKU: A85388
Price: 185.0 USD
Availability: InStock

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$185

Anticorps monoclonal [5J11] de souris contre Actin pour WB, ICC/IF et IHC.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

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Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Mots clés :

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Spécifications

Nom du produit

Anti-Actin Antibody [5J11]

Description du produit

Mouse monoclonal (5J11) antibody to Actin.

Spécificité

This antibody binds all six Actin gene products; not unexpected given the 94-97% sequence identity of the six proteins. As a result this antibody will detect all Actin proteins present in any mammalian cell or tissue extract, making it a useful and versatile western blot loading control. This antibody also works in immunocytochemical experiments, strongly labeling filopodia, membrane ruffles, and stress fibers in cells, all known to be rich in Actin.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:500-1:1,000, IHC: 1:500-1:1,000

Reactivité

Human, Horse, Bovine, Porcine, Rat, Mouse

Immunogène

Actin preparation derived from bovine brain.

Hôte

Mouse

Clonalité

Monoclonal

Clone

5J11

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Masse moléculaire

42 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

a actin, ACTA, ACTA1, Actin alpha skeletal muscle, actin, alpha 1, skeletal muscle, actin, alpha 1, skeletal muscle 1, Actin, alpha skeletal muscle, actina, actine, ACTS_HUMAN, aktin, Alpha Actin 1, alpha skeletal muscle, Alpha skeletal muscle Actin, alpha-actin, Alpha-actin-1, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3, nemaline myopathy type 3

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Immunofluorescence - Anti-Actin Antibody [5J11] (A85388)

Immunofluorescent analysis of HeLa cells stained with Anti-Actin Antibody (green) and Anti-Vimentin Antibody (A85421 | red). The Anti-Actin Antibody labels the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. The Anti-Vimentin Antibody stains a different cytoskeletal network, the intermediate or 10nm filaments. The blue is DAPI staining of nuclear DNA.

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Figure 2: Western Blot - Anti-Actin Antibody [5J11] (A85388)

Western blot analysis of tissue and cell lysates probed with Anti-Actin Antibody (red): [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The same blot was simultaneously probed with Anti-UCHL1 Antibody (A85349 | green) - a marker of neuronal lineage cells.

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Figure 3: Western Blot - Anti-Actin Antibody [5J11] (A85388)

Western blot of crude extract of the human carcinoma HeLa cell line. Lane 15 was probed with Anti-Actin Antibody giving a strong clean band at an SDS-PAGE molecular weight of 42 kDa. Lane 16 was probed with Anti-14-3-3 eta Antibody (A85361) which has an SDS-PAGE molecular weight of 28 kDa. Lanes 17 and 18 were probed with two Anti-Vimentin Antibodies (A85424 & A85423, respectively). Note that both Anti-Vimentin Antibodies show strong clean bands at the expected molecular weight of 50 kDa.