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Anti-Alkaline Phosphatase, Tissue Non-Specific Anticorps (A91218)

Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.com
$335
Product Datasheet

Anticorps polyclonal de lapin contre Alkaline Phosphatase, Tissue Non-Specific pour WB et IHC.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

Nom du produit
Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody
Description du produit
Rabbit polyclonal antibody to Alkaline Phosphatase, Tissue Non-Specific.
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 230-440 of human Alkaline Phosphatase (ALPL) (NP_000469.3).
Séquence
NKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLTLDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHEGKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDKKPFTAILYGNGPGYKVVGGERENVSMVDYAHNN
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
80 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
AKP2, Alkaline phosphatase liver/bone/kidney, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphomonoesterase, Alpl, AP TNAP, AP-TNAP, APTNAP, BAP, FLJ40094, FLJ93059, Glycerophosphatase, HOPS, Liver/bone/kidney type alkaline phosphatase, MGC161443, MGC167935, PHOA, PPBT_HUMAN, Tissue non specific alkaline phosphatase, Tissue nonspecific ALP, TNAP, TNSALP
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Western blot analysis of extracts of HeLa cells, using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Alkaline Phosphatase, Tissue Non-Specific Antibody (A91218) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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