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Anti-alpha 1 Fetoprotein Anticorps [AMC0201] (A308568)

Western Blot - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) - Antibodies.com Immunohistochemistry - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) - Antibodies.com
Ce produit est discontinué

Anti-alpha 1 Fetoprotein Anticorps [AMC0201] (A308568) a été abandonné et n'est plus disponible.

Voir toutes Anti-alpha 1 Fetoprotein Anticorps.

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Nom du produit
Anti-alpha 1 Fetoprotein Antibody [AMC0201]
Description du produit
Mouse monoclonal [AMC0201] antibody to alpha 1 Fetoprotein.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 19-609 of human Alpha-Fetoprotein (AFP).
Séquence
RTLHRNEYGIASILDSYQCTAEISLADLATIFFAQFVQEATYKEVSKMVKDALTAIEKPTGDEQSSGCLENQLPAFLEELCHEKEILEKYGHSDCCSQSEEGRHNCFLAHKKPTPASIPLFQVPEPVTSCEAYEEDRETFMNKFIYEIARRHPFLYAPTILLWAARYDKIIPSCCKAENAVECFQTKAATVTKELRESSLLNQHACAVMKNFGTRTFQAITVTKLSQKFTKVNFTEIQKLVLDVAHVHEHCCRGDVLDCLQDGEKIMSYICSQQDTLSNKITECCKLTTLERGQCIIHAENDEKPEGLSPNLNRFLGDRDFNQFSSGEKNIFLASFVHEYSRRHPQLAVSVILRVAKGYQELLEKCFQTENPLECQDKGEEELQKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTKKAPQLTSSELMAITRKMAATAATCCQLSEDKLLACGEGAADIIIGHLCIRHEMTPVNPGVGQCCTSSYANRRPCFSSLVVDETYVPPAFSDDKFIFHKDLCQAQGVALQTMKQEFLINLVKQKPQITEEQLEAVIADFSGLLEKCCQGQEQEVCFAEEGQKLISKTRAALGV
Hôte
Mouse
Clonalité
Monoclonal
Clone
AMC0201
Isotype
IgG1
Chaînes légères
kappa
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
69 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Afp, AFPD, Alpha fetoglobulin, Alpha fetoprotein, Alpha fetoprotein precursor, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein, alpha-fetoprotein, Hereditary persistence of, included, FETA, FETA_HUMAN, Hereditary persistence of alpha fetoprotein, HPAFP
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)
Western blot analysis of extracts of HeLa cells, using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at 1:1,000 dilution. The secondary antibody was HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568)
Immunohistochemistry analysis of paraffin-embedded mouse fetal liver using Anti-alpha 1 Fetoprotein Antibody [AMC0201] (A308568) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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