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Anti-alpha smooth muscle Actin Anticorps [ARC0298] (A307100)

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Western Blot - Anti-alpha smooth muscle Actin Antibody (A82445)
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Anti-alpha smooth muscle Actin Anticorps
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Western Blot - Anti-alpha smooth muscle Actin Antibody (A82445)
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Anti-alpha smooth muscle Actin Anticorps
Anticorps polyclonal de chèvre contre alpha smooth muscle Actin pour ELISA et WB.
Immunohistochemistry - Anti-alpha Smooth Muscle Actin Antibody - Antibodies.com (A8234)
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Anti-alpha Smooth Muscle Actin Anticorps [M16-L]
Anticorps monoclonal [M16-L] de lapin contre alpha Smooth Muscle Actin pour IHC-P et IHC-Fr.
Nom du produit
Anti-alpha smooth muscle Actin Antibody [ARC0298]
Description du produit
Rabbit monoclonal [ARC0298] antibody to alpha smooth muscle Actin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human a-Smooth Muscle Actin (ACTA2) (P62736).
Séquence
MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAP
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0298
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
42 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
a actin, AAT6, ACTA2, ACTA_HUMAN, Actin alpha 2 smooth muscle aorta, Actin aortic smooth muscle, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha 2 actin, Alpha actin 2, Alpha cardiac actin, alpha sma, Alpha-actin-2, Cell growth inhibiting gene 46 protein, Cell growth-inhibiting gene 46 protein, GIG46, Growth inhibiting gene 46, MYMY5
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Western blot analysis of extracts of various cell lines, using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunofluorescence analysis of smooth muscle of human colon carcinoma using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100)
Immunofluorescence analysis of mouse colon using Anti-alpha smooth muscle Actin Antibody [ARC0298] (A307100) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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