Anti-ATG12 Anticorps [ARC2663] (A309284)

Name: Anti-ATG12 Antibody [ARC2663]
Brand: Antibodies.com
SKU: A309284

Cliquez pour ouvrir le point de vue élargi

Ce produit est discontinué

Anti-ATG12 Anticorps [ARC2663] (A309284) a été abandonné et n'est plus disponible.

Voir toutes Anti-ATG12 Anticorps.

Spécifications

Nom du produit

Anti-ATG12 Antibody [ARC2663]

Description du produit

Rabbit monoclonal [ARC2663] antibody to ATG12.

Applications

WB, IHC, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivité

Human, Mouse

Immunogène

A synthesized peptide derived from human ATG12.

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC2663

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

55 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Apg12, APG12-like, APG12L, ATG12 autophagy related 12 homolog, ATG12 autophagy related 12 homolog (S. cerevisiae), ATG12_HUMAN, Autophagy 12, Autophagy-related protein 12, FBR93, HAPG12, Ubiquitin-like protein ATG12

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)

Western blot analysis of various lysates, using Anti-ATG12 Antibody [ARC2663] (A309284) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunohistochemistry - Anti-ATG12 Antibody [ARC2663] (A309284)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-ATG12 Antibody [ARC2663] (A309284)

Immunoprecipitation analysis of 300µg extracts of 293T cells using 3µg of Anti-ATG12 Antibody [ARC2663] (A309284). This Western blot was performed on the immunoprecipitate using Anti-ATG12 Antibody [ARC2663] (A309284) at a dilution of 1:500.

Produits alternatifs à Anti-ATG12 Anticorps [ARC2663] (A309284)

(2)

A121605

1 Référence