Anti-ATG7 Anticorps [ARC2631] (A306024)

Name: Anti-ATG7 Antibody [ARC2631]
Brand: Antibodies.com
SKU: A306024

Cliquez pour ouvrir le point de vue élargi

Ce produit est discontinué

Anti-ATG7 Anticorps [ARC2631] (A306024) a été abandonné et n'est plus disponible.

Voir toutes Anti-ATG7 Anticorps.

Spécifications

Nom du produit

Anti-ATG7 Antibody [ARC2631]

Description du produit

Rabbit monoclonal [ARC2631] antibody to ATG7.

Applications

WB, IHC, IP

Recommander des dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, IP: 1:100-1:500

Reactivité

Human

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 600-703 of human ATG7 (O95352).

Séquence

GGYAIASSSDDRMNEPPTSLGLVPHQIRGFLSRFDNVLPVSLAFDKCTACSSKVLDQYEREGFNFLAKVFNSSHSFLEDLTGLTLLHQETQAAEIWDMSDDETI

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC2631

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

75 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

1810013K23Rik, Apg 7, Apg7, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7 like, APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG 7, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), Atg7l, ATG7_HUMAN, Autophagy 7, S. cerevisiae, homolog of, Autophagy related protein 7, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, GSA7, hAGP7, Ubiquitin activating enzyme E1 like protein, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

Cliquez pour ouvrir le point de vue élargi

Figure 1: Western Blot - Anti-ATG7 Antibody [ARC2631] (A306024)

Western blot analysis of extracts of HepG2 cells, using Anti-ATG7 Antibody [ARC2631] (A306024) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Cliquez pour ouvrir le point de vue élargi

Figure 2: Immunohistochemistry - Anti-ATG7 Antibody [ARC2631] (A306024)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-ATG7 Antibody [ARC2631] (A306024) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Cliquez pour ouvrir le point de vue élargi

Figure 3: Western Blot - Anti-ATG7 Antibody [ARC2631] (A306024)

Immunoprecipitation analysis of 300µg extracts of HepG2 cells using 3µg of Anti-ATG7 Antibody [ARC2631] (A306024). This Western blot was performed on the immunoprecipitate using Anti-ATG7 Antibody [ARC2631] (A306024) at a dilution of 1:500.