Anti-Aurora A + B + C Kinase Anticorps [4A7] (A85401)

Name: Anti-Aurora A + B + C Kinase Antibody [4A7]
Brand: Antibodies.com
SKU: A85401
Price: 185.0 USD
Availability: InStock

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$185

Anticorps monoclonal [4A7] de souris contre Aurora A pour WB, ICC/IF et IHC.

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Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 4-6 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

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Qualité 100 % Garantie

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Spécifications

Nom du produit

Anti-Aurora A + B + C Kinase Antibody [4A7]

Description du produit

Mouse monoclonal (4A7) antibody to Aurora A + B + C Kinase.

Spécificité

This antibody was raised against human Aurora A protein and was shown to also bind Aurora B and Aurora C; as a result the epitope is likely within the highly conserved serine/threonine kinase domain. This antibody can be used to identify dividing or soon to be dividing cells and is also an excellent marker of midbodies both during and after cell division.

Applications

WB, ICC/IF, IHC

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:1,000-1:2,000, IHC: 1:1,000-1:2,000

Reactivité

Human, Horse, Bovine, Porcine, Rat, Mouse

Immunogène

Recombinant full-length human Aurora A protein, expressed in and purified from E. coli.

Hôte

Mouse

Clonalité

Monoclonal

Clone

4A7

Isotype

IgG1

Conjuguer

Unconjugated

Purification

Immunogen affinity purification.

Concentration

1 mg/ml

Masse moléculaire

46kDa, 38kDa, 35kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 5mM Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

AIK, ARK-1, ARK1, AURA, Aurka, Aurora 2, Aurora kinase A, Aurora-related kinase 1, Aurora/IPL1 like kinase, Aurora/IPL1-related kinase 1, AURORA2, Breast tumor-amplified kinase, BTAK, hARK1, IAK, IPL1 related kinase, MGC34538, OTTHUMP00000031340, OTTHUMP00000031341, OTTHUMP00000031342, OTTHUMP00000031343, OTTHUMP00000031344, OTTHUMP00000031345, OTTHUMP00000166071, OTTHUMP00000166072, PPP1R47, Protein phosphatase 1, regulatory subunit 47, Serine/threonine kinase 15, Serine/threonine kinase 6, Serine/threonine-protein kinase 15, Serine/threonine-protein kinase 6, Serine/threonine-protein kinase aurora-A, STK15, STK6, STK6_HUMAN, STK7

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (2)

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Figure 1: Immunofluorescence - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

HeLa cell cultures were stained with Anti-Aurora A/B/C Kinase Antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Cells were counterstained with Anti-Vimentin Antibody (A85421 | red). Blue is a DNA stain.

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Figure 2: Western Blot - Anti-Aurora A + B + C Kinase Antibody [4A7] (A85401)

Left: Western blot analysis of Anti-Aurora A/B/C Kinase Antibody in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Anti-Aurora A/B/C Kinase Antibody. Nocodazole is a microtubule depolymerizating agent which induces cells to halt at the G2/M phase and also induces Aurora kinase expression. The Anti-Aurora A/B/C Kinase Antibody binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with Anti-Aurora A/B/C Kinase Antibody. This antibody reacts strongly with all three Aurora kinases proteins.