Anti-Bax Anticorps [ARC5006-06] (A306011)

Name: Anti-Bax Antibody [ARC5006-06]
Brand: Antibodies.com
SKU: A306011

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Ce produit est discontinué

Anti-Bax Anticorps [ARC5006-06] (A306011) a été abandonné et n'est plus disponible.

Voir toutes Anti-Bax Anticorps.

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A96467

Anti-BAX Anticorps

Anticorps polyclonal de lapin contre BAX pour IHC, IF et ELISA.

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A96467

Anti-BAX Anticorps

Anticorps polyclonal de lapin contre BAX pour IHC, IF et ELISA.

Spécifications

Nom du produit

Anti-Bax Antibody [ARC5006-06]

Description du produit

Rabbit monoclonal [ARC5006-06] antibody to Bax.

Applications

WB, IHC, ICC/IF, IP

Recommander des dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:50-1:100

Reactivité

Human, Mouse, Rat

Immunogène

A synthetic peptide corresponding to a sequence within amino acids 10-70 of human Bax (NP_620116.1).

Séquence

GGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDEL

Hôte

Rabbit

Clonalité

Monoclonal

Clone

ARC5006-06

Isotype

IgG

Conjuguer

Unconjugated

Purification

Affinity purification.

Masse moléculaire

21 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

Apoptosis regulator BAX, Bax-protein, BAXA, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, BAX_HUMAN, Bcl-2-like protein 4, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, Bcl2-L-4, BCL2L4, membrane isoform alpha

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-Bax Antibody [ARC5006-06] (A306011)

Western blot analysis of extracts from wild type (WT) and Bax knockout (KO) 293T cells, using Anti-Bax Antibody [ARC5006-06] (A306011) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Western Blot - Anti-Bax Antibody [ARC5006-06] (A306011)

Western blot analysis of extracts of various cell lines, using Anti-Bax Antibody [ARC5006-06] (A306011) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 3: Immunohistochemistry - Anti-Bax Antibody [ARC5006-06] (A306011)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Bax Antibody [ARC5006-06] (A306011) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Bax Antibody [ARC5006-06] (A306011)

Confocal imaging of HeLa cells using Anti-Bax Antibody [ARC5006-06] (A306011), at a dilution of 1:100, (red). The cells were counterstained with Anti-Vimentin Antibody, at a dilution of 1:100, (green). DAPI was used for nuclear staining (Blue). Objective: 60x.