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Anti-Bcl-2 Anticorps [ARC0173] (A306910)

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A86278
16 Références
Anti-Bcl-2 Anticorps [Bcl-2/100]
Anticorps monoclonal [Bcl-2/100] de souris contre Bcl-2 pour Flow Cytometry, IP, WB, IHC-P, IHC-Fr et ICC.
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A86278
16 Références
Anti-Bcl-2 Anticorps [Bcl-2/100]
Anticorps monoclonal [Bcl-2/100] de souris contre Bcl-2 pour Flow Cytometry, IP, WB, IHC-P, IHC-Fr et ICC.
Nom du produit
Anti-Bcl-2 Antibody [ARC0173]
Description du produit
Rabbit monoclonal [ARC0173] antibody to Bcl-2.
Applications
WB, IHC, IP
Recommander des dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200, IP: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human Bcl-2 (P10415).
Séquence
MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFD
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC0173
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
26 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
Apoptosis regulator Bcl 2, Apoptosis regulator Bcl-2, Apoptosis regulator Bcl2, AW986256, B cell CLL/lymphoma 2, B cell leukemia/lymphoma 2, Bcl2, BCL2_HUMAN, C430015F12Rik, D630044D05Rik, D830018M01Rik, Leukemia/lymphoma, B-cell, 2, Oncogene B-cell leukemia 2, PPP1R50, Protein phosphatase 1, regulatory subunit 50
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Western blot analysis of extracts of various cell lines, using Anti-Bcl-2 Antibody [ARC0173] (A306910) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human tonsil (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue (100X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-Bcl-2 Antibody [ARC0173] (A306910)
Immunohistochemistry analysis of paraffin-embedded human appendix tissue (400X) using Anti-Bcl-2 Antibody [ARC0173] (A306910) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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