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Anti-Beclin 1 Anticorps [ARC52858] (A307233)

Nom du produit
Anti-Beclin 1 Antibody [ARC52858]
Description du produit
Rabbit monoclonal [ARC52858] antibody to Beclin 1.
Applications
WB, ICC/IF
Recommander des dilutions
WB: 1:2,000-1:10,000, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human Beclin 1 (NP_003757.1).
Séquence
MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSG
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC52858
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
60 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
APG6, ATG 6, ATG6, ATG6 autophagy related 6 homolog, Bcl-2-interacting protein beclin, Beclin 1 (coiled coil moesin like BCL2 interacting protein), Beclin 1 autophagy related, Beclin-1, Beclin1, BECN 1, Becn1, BECN1_HUMAN, Coiled coil myosin like BCL2 interacting protein, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS 30, VPS30
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233)
Western blot analysis of various lysates, using Anti-Beclin 1 Antibody [ARC52858] (A307233) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Beclin 1 Antibody [ARC52858] (A307233)
Western blot analysis of Rat lung, using Anti-Beclin 1 Antibody [ARC52858] (A307233) at 1:10,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233)
Immunofluorescence analysis of C6 using Anti-Beclin 1 Antibody [ARC52858] (A307233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunofluorescence - Anti-Beclin 1 Antibody [ARC52858] (A307233)
Immunofluorescence analysis of HeLa using Anti-Beclin 1 Antibody [ARC52858] (A307233) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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