Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
B2M, B2MG_HUMAN, Beta 2 microglobin, Beta 2 microglobulin precursor , Beta chain of mhc class 1 proteins , Beta chain of MHC class I molecules, Beta-2-microglobulin form pI 5.3, CDABP0092, Hdcma22p, IMD43
Figure 1: Western Blot - Anti-beta 2 Microglobulin Antibody [ARC0656] (A81075)
Western blot analysis of extracts of various cell lines, using Anti-beta 2 Microglobulin Antibody [ARC0656] (A81075) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-beta 2 Microglobulin Antibody [ARC0656] (A81075) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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